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文献和实验Human Peripheral Blood Mononuclear Cell Preparation
Procedure1. Pour buffy coat (see Hint #1) from the bag into a 125 ml conical polypropylene centrifuge tube and dilute to 90 ml with sterile endotoxin-free PBS.2. Add 15 ml of sterile Dextran/PBS and mix by inverting the tube four to five times
Human Peripheral Blood Mononuclear Cell Preparation
This protocol describes a procedure for isolating human peripheral blood mononuclear cells (lymphocytes and monocytes) from a Buffy Coat (obtained from a blood bank). Platelets, red blood cells, and neutrophils are largely depleted
X10MG For MALDI matrix Nile Red Sigma Aldrich 19123-10MG For fluorescence Imaging
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