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文献和实验SDS-PAGE Western Blotting Protocols
glass plate can be treated with Rain-X to facilitate later removal of the gel. 2. Slide into holder without tightening the screws. 3. Place on pouring stand, making sure that the glass plates are pushed all the way to the bottom before tightening
150 mM NaCl 15ml 5M 1 mM EDTA 1ml 0.5M 1% Triton X 100 5ml 0.1 % Sodium deoxycholate 0.5g 0.1 % SDS 5ml 10%
enough, repeat the procedure.Fingerprinting GelEquipment and materials:Stratagene sequencer - CastAwayTM gel system with gasket lubricant(Cat # 401075)Stratagene castAwaytM precast gels (Cat # 401092): 4.5% precast polyacrylamide, 1xTBE, 7 M urea, with 32
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