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文献和实验Materials2 ml size Sarstedt screw cap tubesBIO-RAD Disposable Chromatography Column （BIO-RAD cat. #732-6008）Oligo dT celluloseRNase-free water5.0 M NaCl0.5 M EDTA1.0 M Tris, pH 7.410% SDS70% EtOHBefore starting protocol1. Measure out 75 mg of oligo
Phosphoamino acid analysis:Mark Kamps's method
will work! Keep the membrane wet and wrap in Saran wrap. Add radioactive markings for orientation of filmlater Expose to film. 2.Cut out band of interest,re-wet in methanol,rinse well in water and place in a screw-cap tube containing 150 µl 5.7 NHCl
Analysis of the Protein S Gene in Protein S Deficiency
Protein S (PS) is a 71-kDa vitamin K-dependent glycoprotein first identified in human plasma by DiScipio and colleagues in 1977 (1 ), a year after the discovery of the anticoagulant protein C (PC) (2 ,3 ). A few years later, Walker
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