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文献和实验Partial proteolysis with Staph. aureus V8 protease
. If you have too much, the V8 won't work. The molarity of neat mercaptoethanol is approximately 14. 1 microliter of ME per 14 ml of solution gives a 1 mmolar solution. Enzyme stock solution: 500 micrograms/ml (or 500 nanograms/microliter) V8-protease in 125
Appendix H: Radioactive tracers
. It may have one, two, or three neutrons. The isotopes of hydrogen would be written as H H H (in all further references, the atomic number subscript 1 is left off for clarity). H is the most stable form of hydrogen and is therefore the most abundant (99.985% of all forms). H
1.按“power”键打开离心机电源开关; 2.平衡放置离心管; 3.设置离心参数: (1)调节温度按钮,设定所需离心温度; (2)调节速度设置按钮,设定所需离心速度,但最高不得超过14000rpm; (3)揿分/秒选择按钮,确定使用“分”或“秒”计时,再调节时间设置按钮,设置离心时间; (4)盖上离心机 ,按“start”键,离心机工作; 4.如发现有不平衡或其他异常情况,按“stop”键立即停止离心; 5. 使用完毕,擦干离心机,打开盖子,做好使用记录。 注意: A.
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