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文献和实验(pMDL =0.75 μg, VSV-G=0.45 μg, REV=0.3 μg) add 200 μl OPTI-MEM, mix well. 2. Mix 7.5 μl LF2K in 200 μl OPTI-MEM, incubate for 5 min. 3. Mix the LF2K mixture with plasmids mixture, incubate for 15-20 min. 4. During the incubation time, trypsinize
, and lyophilized as described above, the resulting white powder is dissolved in DMSO to make a 2 mM stock solution. 1) Prepare 500 μL of 20 μM peptoid solution in sheet formation buffer (10 mM Tris-HCl, 100 mM NaCl, pH 8.0 in water) in a 1 dram
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