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文献和实验Transformation of E. coli by Electroporation
autoclaved bottles!). 4. Wash the cell pellet by resuspending in 250 ml of sterile ice-cold WB (10% redistilled glycerol, 90% distilled water, v/v). Centrifuge the cell suspension at 5,000 RPM for 15 min and carefully pour off the supernate as soon
groups, made of regular glass, Corex glass, nitrocellulose, or polyallomer. Regular glass centrifuge tubes can be used at speeds below 3,000 RPM, that is in a standard clinical centrifuge. Above this speed, the xg forces will shatter the glass
without external tubes. For biological work, the tubes are divided into functional groups, made of regular glass, Corex glass, nitrocellulose, or polyallomer. Regular glass centrifuge tubes can be used at speeds below 3,000 RPM, that is in a standard clinical
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