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文献和实验因为自己实验后期需要使用这方面的技术,所以最近在查阅这方面的资料,想拿出来和大家一起分享.后期会把更详细的东西贴出来.英文水平一般,所以翻译的有点差,请海涵. Rac1 Activation Assay Kit被设计来鉴定贴壁细胞或非贴壁细胞的细胞裂解液中Rac活性数量(i.e. Rac-GTP, rather than the inactive Rac-GDP). 使用的glutathione (GST) pull-down方法,采用GST-agarose beads与有的包含74
Immobilization of Enzymes on Magnetic Beads Through Affinity Interactions
in analytical assay because magnetic beads provide a relative large numbers of binding sites for biochemical reactions resulting in faster assay kinetics. This chapter describes immobilization procedures of tagged enzymes onto various magnetic
DMB (DNMT-Magnetic Beads) Assay: Measuring DNA Methyltransferase Activity In Vitro
a novel assay system, in which the activity of a DNA methyltransferase is measured as the incorporation of tritium into biotinylated DNA oligonucleotides. The DNA is immobilized onto magnetic beads with streptavidin covalently attached to the bead surface
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