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文献和实验Single Clone Excision Protocol
. 4.In a 15 ml conical tube,place 200 ml XL1-Blue MRF' cells (at A600 of 1.0; if absorbance differs,adjust amount proportionally) 250 ml of phage stock (from step 1 above.Make sure that you don't transfer chloroform from the bottom of the tube). 1 ml
Clone Genes From a Phage Library
much more complicated than it actually is. First you must have the SolR cells grown up and prepared for phage plating, and get out some Exassist phage as well from the freezer. • In a 15 ml falcon tube, combine 200 microliters XL blue cells and 200
Clone information and RNAi Feeding Protocol
AHRINGER LAB Clone information and RNAi Feeding Protocol Vector and inserts: Genomic fragments obtained by PCR were cloned into the Timmons and Fire feeding vector (L4440
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