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- merck-millipore
- HAP0C3-MAG
- 2025年08月18日
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文献和实验the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
antibiotic (see Step 51). Ligation buffer (10X) Dilute the 10X stock to 1.15X for Step 18. Magnetic beads, Protein A-conjugated Magnetic beads, Protein G-conjugated PCR buffer (10X) PCR primers, T3 and T7 Phenol
Native Chromatin Preparation and Illumina/Solexa Library Construction
Immunoprecipitation (ChIP) Do NOT block beads with any nonspecific DNA or RNA. 30. Place 40 µL of Dynabeads Protein A into each of two microcentrifuge tubes. Wash each with 600 µL of 1X PBS. Place the tubes in a magnetic stand and aspirate
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