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文献和实验DNA Transfection of Mammalian Cells Using Polybrene
products and to clone their genes (5 ), the reduced ability to transform CHO cells by the calcium phosphate technique has slowed the use of the large number of CHO cell mutants (6 ) in transfection and rescue experiments. In this chapter
Cationic Liposome-Mediated Transfection with Lipofectin Reagent
Since their original description, liposomes have been discussed as vehicles that could be used as carriers of pharmaceutically active agents (1 ), and their potential for use as carriers of genetic information has been examined
Polybrene/DMSOAssisted Gene Transfer
Efforts to expand the current repertory of cell types amenable to transfection have often been thwarted by a common obstacle-namely, the low tolerance displayed by the recipient cells toward the gene-transfer regimen itself. As a result
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