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- merck-millipore
- SLLG013SL
- 2025年08月04日
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文献和实验Culturing Chick Embryonic Myoblasts/Myotubes and Fibroblasts
3. Afterwards replace medium every third day (no ara c). Ara C Stock (1 mM) 1. Add 1 mg ara c to 2.5 ml distilled H2O. 2. Sterile filter through Millex-GV filter. 3. Store in freezer (-20o C). 4. Use at 5 x 10-6 M in regular skeletal muscle
In situ hybridisation to alpha satellite sequences (chromosome specific)
; 100% ethanol 2 x 5 minutes, 90% Ethanol 5 minutes, 75% ethanol 5 minutes, H2 O 2 x 5 minutes and allow to dry. Store dry, dust-free. Typical Protocol (1) Immerse the slides in 1M sodium thiocyanate at 80C for 10 minutes. Rinse in H2 O and allow
(100 μg/ mL) Adjust the pH of the resulting solution to 7.2 with the help of 1N NaOH and sterilize through 0.22 μm Millex-GV filter unit (Millipore). Store the prepared medium in small aliquots at 5° C in dark. Erythrocyte lysis buffer
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