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- merck-millipore
- SCSZT0020
- 2025年07月30日
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文献和实验BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
X75 mm Falcon tube containing 3 ml of LB medium (10 g Bacto-Tryptone, 5 g Bacto-yeast extract, and 10 g NaCl in 1 L H2O, autoclaved) with appropriate antibiotic. After incubating at 37 degrees C for 8-10 hours with 250 rpm shaking, the culture
Single tube confirmation PCR protocol
(200mg/l). - Incubate at 30°C for two to three days. *This step reduces background by eliminating aborted transformants. 2. Zymolyase treatment - Transfer a small portion of a well-isolated colony (~1 mm) into 50 µl solution
by pipetting them up and down three times. Transfer 200 ul of the mixture to the 48-well plate (Plate A). 6. Incubate at 37 degreeC, 5% CO2 for 3 to 30 h. 7. Day 2 and 3: Observe endothelial tube size and numbers under microscope and photograph
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