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- 2025年07月24日
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文献和实验The outer layer of human skin is the epidermis. More than 90% of the epidermal cells are keratinocytes, which originate from stem cells, located in the basal cell layer (Stratum basale ). Keratinocytes in the basal layer divide, differentiate
for 10 min and aspirate supernatant. Resuspend in 10ml growth medium and divide evenly between 2x 15ml tubes. Allow to sediment for 10 min and aspirate the supernatant. Using a 1ml barrier tip and Gilson, add 1ml differentiation medium
must be maintained in such a way that they persist in their differentiated state. While many hepatocyte culture methods exist, the two-dimensional collagen “sandwich” system combined with a serum-free medium, supplemented with physiological glucocorticoid
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