Xeno-Free Trypsin/EDTA Kit

Miniprep/Kit-free high-throughput protocol

or 37 ˚C or dry in a speedvac). Rehydrate in 50 μL TE. Buffers STET 8% sucrose 50 mM Tris-HCl, pH 8 0.5% Triton X-100 50 mM EDTA TE 10 mM Tris-HCl, pH 8 1 mM EDTA  

All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies

to obtain a 1X Citrate Buffer, pH 6.0 solution. To 25 ml 20X EDTA solution, pH 8.0, add 475 ml distilled water to obtain a 1X EDTA solution, pH 8.0. 3 ） Place the slides in a slide rack and place the rack in a 1 L glass beaker containing

E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol

on desired concentration of final product) Endotoxin-Free Elution Buffer directly onto the column matrix and let it sit at room temperature for 2 minutes. Centrifuge at 13,000 x g for 1 min to elute DNA. This represents approximately 65-85% of bound DNA