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- merck-millipore
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- 2025年07月10日
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
dense products have been devised. A chromogenic substrate, 5-bromo-4-chloro-3-indolyl phosphate (X-Phos), which is very similar to X-gal, can be employed for detection and leads to production of a blue precipitate (Figure 1). In addition, the tetrazolium
96-well RNA In Situ Hybridization Protocol
). To stop development of individual wells, remove staining solution and add 200ul PBT. 26. To stop entire plate, rinse plate 3X with PBT. 27. Add 200ul 70% gycerol to each well. 28. Embryos are ready to mount or dissect once they have settled
Cell cycle analysis of Escherichia coli cells
: The C+D period is estimated from the initiation age (ai ), the generation time (τ) and the number of generations spanned per cell cycle. Example: 4-origin-cells: 23 % Generation time (τ): 27 min Initiation age (ai ): 5 min
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