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文献和实验Study of Substrate Specificity of MAPKs Using Oriented Peptide Libraries
, particularly mass spectroscopy, has greatly facilitated this analysis (1 –3 ), although usually the investigator must still perform large scale biochemical purification of the presumed substrate to determine its identity. The use of oriented peptide library
Two dimensional peptide mapping
This specfic protocol is the latest incarnation of peptide mapping procedures that have been developed here in the TVL/MBVL of the Salk Institute over the last 20 years. Wade Gibson developed the first peptide mapping protocol
Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library
We have identified a peptide substrate for molecular chaperone Hsp40 Ydj1 by utilizing the combination of phage display library screening and isothemol titration calirimetry (ITC). The initial peptide substrate screening for Hsp40 Ydj
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