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北京盛科博源生物科技有限公司
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文献和实验• stabilizer soln (10% sodium thiosulfate+0.1N NaOH) • IODO-BEAD™(PIERCE) • Hamilton microsyringe (model 702, 25µl, needle gauge 22S, point style #2) • desalting column (Bio-rad DG-10 etc.) • column stand For procedure 2 • PBS
reactivity function forms an irreversible covalent bond to the target protein upon irradiation, and a sorting function allows the captured protein(s) to be isolated and identified by mass spectrometric analysis in an affinity-driven manner. Capture Compounds™
Isolate It All:siRNA • miRNA • Total RNA • Native Protein
with ethidium bromide. After electrotransfer, miR-16 miRNA was detected by Northern blot with an antisense RNA probe 5' labeled and purified with the mir Vana™ Probe & Marker Kit (Ambion). Analyze siRNA, mRNA, and Protein Expression in RNAi
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