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文献和实验Clone Genes From a Phage Library
per ml as well). Both of these strains should be recovered from the freezer onto the appropriate drug plate and then kept in the refrigerator. Grow the bacteria in LB 0.2 percent maltose (to express the mal permease which is the lambda receptor) overnight
Construction and Manipulation of Large-Insert Bacterial Clone Libraries
Introduction Construction of Large-Insert Bacterial Clone Libraries Fig. 1. A general procedure
Clone Genes From a Phage Library
(use 25 micrograms per ml as well). Both of these strains should be recovered from the freezer onto the appropriate drug plate and then kept in the refrigerator. Grow the bacteria in LB + 0.2 percent maltose (to express the mal permease
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