FITC-DEXTRAN SOLUTION 10ML

Mag-Bind Blood DNA Maximum Yield Protocol (7ml-10ml)

temperature by incubating at room temperature for 5 minutes. 5. Add indicated volume of absolute ethanol showed in the table to the lysate. Mix throughly by inverting or voretxing. 6. Add 300ul Mag-Bind Particles Solution C to the sample. Mix

人单核细胞和中性粒细胞

Purpose Materials10ml 6% dextran + 7ml citrate/citric acidDextran: T500 --> 6g+100ml PBSCitrate solution: 25g Na Citrate + 8g citric acid + 500 ml PBS 43 ml blood 12 ml RT Histopaque 107718 ml cold H2 O2 ml 10x PBSM199 for HUVECs: 1L powder pocket

原创：western protocol

mM TrisCl 6.057g Chromogenic solution 33ulNBT solution 33ulBCIP solution 10ml alkaline phosphatese buffer NBT solution:0.1g NBT in 10ml 70% N,N-dimethylformamide BCIP solution:0.5g BCIP in 10ml N,N-dimethylformamide Alkaline Phosphatese buffer 1L