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- merck-millipore
- 9000-4LCN
- 2025年07月16日
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文献和实验Clonality - X Chromosome Inactivation Assay
(1:1:1, v:v:v) (Gibco) 3 M Na Acetate, pH 5.2 (Life Technologies) Isopropanol Glycogen, 10 mg/ml (GenHunter) 70% ethanol Buffer 1 (50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 50 mM NaCl) Hha I restriction enzyme (Life Technologies) REact 2 Buffer
Reaction Mixture as follows: 7. Boil reaction in heating block for 5 min. Note: be sure block wells are filled with H2O. 8. Centrifuge briefly to collect condensation. 9. Add 10μl 5X prime dCTP buffer to reaction mix. 10. Add 5μl labeled
Preparation of Microinjection Buffer
Buffer composition is 10 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 30 microM spermine, 70 microM spermidine, 100 mM NaCl. This buffer is more likely to produce transgenic mice with intact, unfragmented DNA molecules. See the following references: Schedl
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