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- merck-millipore
- 71505-3CN
- 2025年07月14日
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
overnight or -70°C for 15 min. 13. Spin 12000g, 10 min, 4°C. Wash pellet with 70 EtOH and drain. Pellet should be a light pink or white. 14. Resuspend in 0.4 ml sterile gd H2 O in a microfuge tube. Precipitate with NaOAc and EtOH as in step 12.
Fast and reliable mini-prep RNA extraction from Neurospora crassa
Eppendorf tube. The tube can be filled with powdered mycelium - Shake for 15-20 min (Eppendorf Rotationsmischer 3300) - Centrifuge for 10 min, 10,000 rpm - Transfer the upper phase into an equal volume of phenol (saturated with 0.1 M Tris HCl, pH
to an eppendorf tube and microfuge at 12,000 rpm for 1 min. 2. Resuspend the bacterial pellet in 250 µl of Buffer P1. 3. Add 250 µl of Buffer P2, mix gently, and incubate at room temperature for 5 min. 4. Add 350 µl of N3, mix thoroughly but gently
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