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北京盛科博源生物科技有限公司
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文献和实验Caulimovirus Isolation and DNA Extraction
Members of the caulimovirus group (1 ) each have a circular double-stranded DNA genome of approx 8 kbp that is encapsidated in a spherical, naked nucleocapsid of approx 50 nm diameter (Fig. 1 ). Caulimoviruses characteristically produce
OGMP - Organelle DNA Library Construction
(version MAY-1998) I. NEBULIZATION of DNA 1. 0.5 - 5 ug DNA in TE (10mM/1mM), 25% glycerol, final volume 500 ul 2. nebulize for 90-100 sec at 5-10 psi (for a medium size of about 1.5kbp) 3. Precipitate with EtOH/Ammonium
I. NEBULIZATION of DNA 1. 0.5 - 5 ug DNA in TE (10mM/1mM), 25% glycerol, final volume 500 ul 2. nebulize for 90-100 sec at 5-10 psi (for a medium size of about 1.5kbp) 3. Precipitate with EtOH/Ammonium Acetate
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