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文献和实验Lentiviral Vector Gene Transfer into Human T Cells
Efficient gene transfer into T lymphocytes may allow the treatment of several genetic dysfunctions of the hematopoietic system, such as severe combined immunodeficiency, and the development of novel therapeutic strategies for diseases
Purification of the T Cell Antigen Receptor and Analysis by Blue-Native PAGE
The T cell antigen receptor (TCR) is a multi-protein complex composed of six different transmembrane subunits, which form complexes of various sizes on the surface of resting T cells. The stoichiometry of the smallest form was recently
Reaction Mix: It is again convenient to make up a master mix to be aliquotted out to amplify all samples, if they are to use the same primers. If not, modify master mix by simply leaving out primers. Total volume: 50ul / rxn x N + 5-10
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