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北京盛科博源生物科技有限公司
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文献和实验This chapter introduces a new method of maintaining human embryonic stem cells (hESCs) in the undifferentiated state through treatment with a GSK-3 inhibitor, BIO, under a feeder-free condition. Additionally, methods are introduced that determine
Expanding Mouse Ventricular Cardiomyocytes Through GSK‐3 Inhibition
.9.1?23.9.10. © 2013 by John Wiley & Sons, Inc. Keywords: cardiomyocyte proliferation; differentiation; isolation; expansion; GSK?3 inhibitor; Wnt/??catenin signaling
to an increase of β-catenin expression easily detectable by Westernanalysis. To find out the optimal concentration of GSK-3 inhibitor in cells of interest at certain time point, cells are treatedwith different concentration of drug (e.g., AR-A014418 as presented
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