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文献和实验【求助】求助!!关于EMSA核蛋白提取过程中用到的100x Protease Inhibitor
要购买,而分别购买这些试剂的钱加起来可以买个核蛋白提取试剂盒了很不划算。有没有做EMSA的人啊,你们提核蛋白的Protease Inhibitor都怎么来的? 有人用过上海生工的核蛋白提取试剂盒了,那个只要250元,提出来的效果怎么样? xhjggl 效果比较好,他们也有100x Protease Inhibitor卖 ,打折卖100x1ml 525元 月下独见 谢谢LS,这样看直接买核蛋白提取试剂盒还划算
Purification of Inhibitor-Free Soil DNA Obtained by other Methods
实验步骤 1. Dissolve the DNA pellet with 200 ul of Elution Buffer or Buffer TE. Important: DNA pellet should be dissolved in Elution Buffer or Buffer TE(pH8.0). 2. Add 50 ul inhibitor Removal Resin to the sample
(Table 1 ). The enzymatic activity of MMP family members is controlled by a group of inhibitor proteins known as the Tissue Inhibitors of Metalloproteinases (TIMPs), which consist of four family members (Table 2 ). Whereas all four TIMPs can inhibit
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