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文献和实验The Detection of Neomycin Phosphotransferase Activity in Plant Extracts
The selectable marker gene so far most frequently used in plant transformation experiments is the well-known npt II or neomycin phosphotransferase II gene, also referred to as aph (3′)II or aminoglycoside phosphotransferase II (1 ). Derived
Metabolic Labeling with Sulfate
of proteins make it possible to determine where a protein normally resides or to follow its transport through the cell. One such modification is addition of sulfate either to tyrosine residues or to carbohydrate side chains. Labeling studies with [35 S
Ammonium Sulfate Binding Assay for Cells
1. Resuspend cells (vegetative or developed) in phosphate buffer at 1 x 10 8 /ml . 2. Add 50 ul cells to 50 ul 20 nM 3H-cAMP in 5-10 mM DTT (for background binding, add same amount of hot cAMP plus 10 uM cold cAMP) in 1.5 ml eppendorf tube
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