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文献和实验A method to re-PCR unique bands from products of mixed size INTRODUCTION The products of a PCR reaction - especially when this is done on eukaryotic genomic DNA, and when using degenerate primers - often contain a mixture of discrete-sized bands
Extraction and PCR Analyis of DNA from Mice Tails
Place numbered Eppendorf tubes in dry ice. Cut 0.5cm from end of tails and place in tubes.Store at -80℃ until analyzed. Add 0.3ml of STE/PK solution to the Eppendorf tubes. Incubate at 55℃ overnight with rocking or for several hours
A method to re-PCR unique bands from products of mixed size Contents INTRODUCTION PROTOCOL COMMENTS INTRODUCTION The products of a PCR reaction - especially when this is done on eukaryotic genomic DNA
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