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文献和实验Shaker to produce the "starter culture". Add 1.8 ml of TB medium to each well in the 96 deep well block. Dilute culture 100 fold with TB and innoculate 5 μl of the diluted culture into each well using the Eppendorf repeater pipette. Grow the BAC
to each well in the 96 deep well block. Dilute culture 100 fold with TB and innoculate 5 μl of the diluted culture into each well using the Eppendorf repeater pipette. Grow the BAC clone in the Floor Shaker for 18 hours at 37 degrees C shaking at 350
Embryo Dissection and Micromanipulation Tools
the wax with a warmed pipette tip. Once set, the wax holds the loop in place. A hair loop can also be set in beeswax by dipping it into molten beeswax (melted on a hotplate). After removing the loop from the wax, blot excess wax from the loop
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