- 询价
- P300-510
- 进口
- 2025年11月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
Seebio
- 英文名：
Glutamine, 100X, 200 mM
- 库存：
大量
- 规格：
Box of 50 10 mL tubes
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文献和实验BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
should be frozen and stored at -70 degrees C. 2、Prior to use, the cells from 200 ml growth were thawed and resuspended in 8 ml of just 10 mM of EDTA, pH 8.0 by gently pipetting up and down with a 10 ml pipette do not vortex. Cells should be resuspended
Protocol for isolating DNA from embryo yolk sacs, or toes, or 2 mm tail for PCR
1. Dissect embryos and place each yolk sac in a microfuge tube (can be frozen at -20°C prior to extraction). Original method cited for mouse toes, or 2 mm tail, Reagents: A) 25 mM NaOH / 0.2 mM EDTA. The pH is about 12. B) 40 mM Tris HCl
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
by centrifugation at 5K rpm for 15 minutes in a 250 ml bottle in the RC5-B using the GSA rotor, cell pellets should be frozen and stored at -70 degrees C. 2、Prior to use, the cells from 200 ml growth were thawed and resuspended in 8 ml of just 10 mM of EDTA, pH
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