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- 2025年09月03日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
Seebio
- 英文名：
QuantumRNA™ ClassicII 18S Internal Standards Kit
- 库存：
大量
- 保存条件：
-20C
- 规格：
100 rxns
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文献和实验Use of Internal and External Standards or Reference RNAs for
"housekeeping" gene would appear to be a good model for an internal standard RNA. Unfortunately, there is no one single RNA with a constant expression level in all of these situations (although 18S rRNA appears to come close to being an ideal internal control
Real-Time PCR Quantification Using Cloned Standards and Multiple Housekeeping Genes
are being optimized for each target. Cloning the target sequence using TOPO TA cloning kit is cost effective, convenient, fast (two days) and can easily be done in any laboratory setting. The plasmid inserted with the target sequence can be readily used as standards
A and Mixing primers with competimers at a 3:7 ratio attenuates the 18S rRNA signal, making 18S rRNA a practical internal control (panel C). Ambion's QuantumRNA™ Technology in Multiplex Quantitative RT-PCR using 18S rRNA as an Internal Control. RT
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