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NPW/Neuropeptide W Rabbit Poly

clonal Antibody抗体,orb158025,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb158025
  • 英国
  • 2025年12月03日
  • IF, IHC-Fr, IHC-P
  • Rabbit
  • Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      NPW/Neuropeptide W Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      NPW/Neuropeptide W Rabbit Polyclonal Antibody

    • 靶点

      NPW

    • 浓度

      1mg/ml

    • 应用范围

      IF, IHC-Fr, IHC-P

    • 宿主

      Rabbit

    • 适应物种

      Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb158025

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human Neuropeptide W (33-55/165aa)

    • 规格

      50 ul

    产品描述:NPW/Neuropeptide W Rabbit Polyclonal Antibody

    别名:hPPL8; L8; L8C; Neuropeptide W; PPL8; PPNPW; Preproprotein L8; NPW_HUMAN.

    免疫原:KLH conjugated synthetic peptide derived from human Neuropeptide W (33-55/165aa)

    预测反应性:Bovine, Human, Mouse

    分子量:3 kDa

    应用稀释比例:IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:Q8N729

    Note:For research use only.

    产品细节图片1
    paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Neuropeptide W) Polyclonal Antibody, Unconjugated (orb158025) at 1:400 overnight at 4°C, followed by a conjugated secondary (orb868589) at 1:500 for 90 minutes and DAPI staining of the nuclei.

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    图标文献和实验
    相关实验
    • p27 ELISA

      then serially diluted in 100 µl assay buffer on the plate). Incubate @ RT for 1 hr while shaking. Meanwhile let TMB solution equilibrate to RT. Wash plate 3x and add 100 µl/well rabbit polyclonal anti-p27 antibody diluted 1:1000 in assay buffer

    • Mapping Protein Distributions on Polytene Chromosomes by Immunostaining

      Reagents Ammonium sulfate (50% [w/v]) (optional, see Step 23) Antibodies, primary Affinity-purified primary antibodies are diluted in PBS containing 1% BSA. The dilutions must be adjusted for each individual primary antibody. Typical

    • ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION

      although other techniques can be used. The choice of immobilized antibody binding protein depends upon the species that the antibody was raised in. Protein A binds well to rabbit, cat, human, pig and guinea pig IgG as well as mouse IgG2a and IgG2b . Protein G binds

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