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- Biorbyt
- orb154518
- 英国
- 2026年07月22日
- FC
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- 文献和实验
- 技术资料
- 抗体名：
BrdU Antibody (PE) 抗体
- 抗体英文名：
BrdU Antibody (PE)
- 靶点：
BrdU
- 应用范围：
FC
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb154518
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
PE
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
Mouse IgG1
- 免疫原：
详询
- 规格：
100 T
产品子类型: Primary Antibody
描述: Mouse monoclonal antibody to BrdU (PE )
克隆号: Bu20a
别名: 5-bromodeoxyuridine
应用说明: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
免疫原: Bromodeoxyuridine conjugated to BSA
防腐剂: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
纯化方法: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
特异性: The antibody Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining. It cross-reacts with EdU.
Entrez 基因 ID: 35
保存条件: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
备注: For research use only.
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文献和实验Western Blotting with Horseradish Pe
7.5, 100mM NaCl, 0.1% Tween 20). 2.Incubate the blot for 30 minutes at 37℃, 1 hour at room temperature, or overnight at 4℃. INCUBATION WITH HORSERADISH PEROXIDASE CONJUGATED ANTIBODY NOTE: THE INCLUSION OF SODIUM AZIDE IS TO BE AVOIDED IN ALL STEPS
a variety of cellular sources from human olfactory epithelium (Hahn et al., 2005) and neural progenitors (Nieoullon et al., 2005) to neural stem cells (Chu et al., 2004). BrdU antibody from Abcam Identification of the labelled cells can be applied
Immunohistochemistry and Detection of Proliferating Cells by BrdU
Immunohistochemistry is a classic technique used for the localization of antigenic target molecules in �tissue. The method exploits the principle that the target antigen is recognized by specific antibody and is visualized using different
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