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- 详细信息
- 技术资料
- 抗体名:
VDAC1 Antibody抗体
- 抗体英文名:
VDAC1 Antibody
- 靶点:
VDAC1
- 浓度:
1 mg/ml
- 应用范围:
ICC, IF, IHC, WB
- 宿主:
Mouse
- 适应物种:
Human, Mouse, Rat
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb150784
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Monoclonal
- 亚型:
IgG2a
- 免疫原:
Fusion protein amino acids 1-283 (full-length) of human VDAC1. Mouse: 98% identity (279/283 amino acids identical). Rat: 98% identity (279/283 amino acids identical) > 60% identity with VDAC2 and VDAC3.
- 规格:
100 ug
产品描述:Mouse Anti-Human VDAC1 Monoclonal IgG2a
别名:Voltage Dependent Anion Channel 1, Porin, Voltage dependent anion selective channel protein 1, Voltage-dependent anion-selective channel protein 1, hVDAC1, MGC111064, Mitochondrial Porin, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, PORIN-31-HL, VDAC 1, VDAC, VDAC-1
免疫原:Fusion protein amino acids 1-283 (full-length) of human VDAC1. Mouse: 98% identity (279/283 amino acids identical). Rat: 98% identity (279/283 amino acids identical) > 60% identity with VDAC2 and VDAC3.
克隆性:N152B/23 (Formerly sold as S152B-23)
分子量:30kDa
应用稀释比例:WB (1:1000)
应用注释:1 µg/ml of SMC-456 was sufficient for detection of VDAC1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
防腐剂:PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer changes when conjugated
纯化:Protein G Purified
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
NCBI:NP_003365.1
Entrez:7416
UniProt ID:P21796
Note:For research use only.


别名:Voltage Dependent Anion Channel 1, Porin, Voltage dependent anion selective channel protein 1, Voltage-dependent anion-selective channel protein 1, hVDAC1, MGC111064, Mitochondrial Porin, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, PORIN-31-HL, VDAC 1, VDAC, VDAC-1
免疫原:Fusion protein amino acids 1-283 (full-length) of human VDAC1. Mouse: 98% identity (279/283 amino acids identical). Rat: 98% identity (279/283 amino acids identical) > 60% identity with VDAC2 and VDAC3.
克隆性:N152B/23 (Formerly sold as S152B-23)
分子量:30kDa
应用稀释比例:WB (1:1000)
应用注释:1 µg/ml of SMC-456 was sufficient for detection of VDAC1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
防腐剂:PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer changes when conjugated
纯化:Protein G Purified
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
NCBI:NP_003365.1
Entrez:7416
UniProt ID:P21796
Note:For research use only.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VDAC1 Monoclonal Antibody, Clone N152B/23. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VDAC1 Antibody (D) Composite.

Western Blot analysis of Mouse Brain and Human RT-4 lysates showing detection of ~30 kDa VDAC1 protein using Mouse Anti-VDAC1 Monoclonal Antibody, Clone N152B/23. Lane 1: Molecular Weight Ladder. Lane A: Mouse Brain Lysate. Lane B: Human RT-4 Lysate. Block: 5% Skim milkin TBST. Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody at 1:200 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: ~30 kDa.
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VDAC1 Antibody抗体,orb150784,biorbyt
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