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文献和实验Conjugation of Antibody to HRP
the first peak. Preparation of Ab:HRP Conjugate Immediately mix the HRP with the thiolated antibody at 1 mg HRP per mg antibody. Gently stir for 16-20 hours at 2-8oC. Dialyze against 3 changed of PBS, changing every 2 hours, 100X the conjugate
All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
for more details. The HRP Polymer Conjugate will react with mouse, rabbit, guinea pig, and rat primary antibodies. 1) Dilute primary antibody in Antibody Diluent as recommended by the antibody manufacturer or as determined by a titration experiment
Conjugation of Antibody to HRP
the first peak. Preparation of Ab:HRP Conjugate Immediately mix the HRP with the thiolated antibody at 1 mg HRP per mg antibody. Gently stir for 16-20 hours at 2-8o C
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