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Kir2.1 Antibody抗体,orb67400,bio

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  • ¥7670
  • biorbyt已认证
  • orb67400
  • 英国
  • 2025年12月04日
  • AM, ICC, IF, IHC, WB
  • Mouse
  • Human, Monkey, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Kir2.1 Antibody抗体

    • 抗体英文名

      Kir2.1 Antibody

    • 靶点

      Kir2.1

    • 浓度

      1 mg/ml

    • 应用范围

      AM, ICC, IF, IHC, WB

    • 宿主

      Mouse

    • 适应物种

      Human, Monkey, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb67400

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Recombinant

    • 亚型

      IgG1

    • 免疫原

      Fusion protein amino acids 41-64 and 189-428 of mouse Kir2.1

    • 规格

      100 ug

    产品描述:Mouse Anti-Mouse Kir2.1 Monoclonal IgG1

    别名:Kir2.1, KCNJ2, Inward rectifier potassium channel 2, Inward rectifier K(+) channel Kir2.1, Potassium channel inwardly rectifying subfamily J member 2, IRK1, HHBIRK1, HHIRK1, HIRK 1, LQT7, SQT3

    免疫原:Fusion protein amino acids 41-64 and 189-428 of mouse Kir2.1

    克隆性:S112

    分子量:45kDa

    应用稀释比例:WB (1:1000), IHC-P (1:1000), ICC/IF (1:100)

    应用注释:1 µg/ml of SMC-310 was sufficient for detection of Kir2.1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    防腐剂:PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

    纯化:Protein G Purified

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    NCBI:NP_032451

    Entrez:16518

    UniProt ID:P35561

    Note:For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir2.1 Monoclonal Antibody, Clone S112. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kir2.1 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kir2.1 Antibody (D) Composite.

    产品细节图片2
    Immunohistochemistry analysis using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear expression in the epidermis and hair follicles.

    产品细节图片3
    Western Blot analysis of Monkey COS transient cell lysate showing detection of Kir2.1 Potassium Channel protein using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

    产品细节图片4
    Immunohistochemistry analysis using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

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      CM1900 cryostat and left to dry before storage at 80°C. Before the application of antibodies to the sections, the tissue was fixed for 20 min with 3%-paraformaldehyde solution. The primary antibodies (anti-Kir2.1, anti-Kir2.3, or anti-Ncadherin

    • 用光改变世界,光遗传学技术原理及光敏蛋白全介绍

      。当把NpHR表达在哺乳动物脑内时,会聚集在内质网上,因此,将内质网输出元件、高尔基体输出元件和来自于钾离子通道Kir2.1的上膜元件加在NpHR基因序列后面,这样就能实现在神经元细胞膜上的高量聚集,修改过的NpHR被称为eNpHR3.0,因此,eNpHR3.0 对细胞膜的靶向性较好,电流较为持久,响应时间短,反应灵敏。 Arch:566nm左右激发。即为archaerhodopsin,是一种黄色激光激活的外向整流质子泵,能够将带正电的质子从神经元内移动到细胞外环境中,使神经元处于超极化状态。在特定

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