- ￥6903
- Biorbyt
- orb67390
- 英国
- 2026年07月24日
- AM, ICC, IF, IHC, IP, WB
- Mouse
- Hamster, Human, Mouse, Rat
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- 文献和实验
- 技术资料
- 抗体名：
Cav1.2 Antibody 抗体
- 抗体英文名：
Cav1.2 Antibody
- 靶点：
Cav1.2
- 浓度：
1 mg/ml
- 应用范围：
AM, ICC, IF, IHC, IP, WB
- 宿主：
Mouse
- 适应物种：
Hamster, Human, Mouse, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb67390
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Recombinant
- 保存条件：
详询
- 亚型：
IgG1
- 免疫原：
详询
- 规格：
100 ug
类别: Antibodies
产品子类型: Recombinant Antibody
描述: Mouse Anti-Rabbit Cav1.2 Monoclonal IgG1
克隆号: S57
别名: Voltage-dependent L-type calcium channel subunit alpha-1C, Cav1.2, Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle, Voltage-gated calcium channel subunit alpha Cav1.2, DHPR alpha-1 subunit, CACH3, CACN4, CACNL1A2, alpha-1 subunit voltage-dependent calcium channel, calcium channel voltage-dependent L type alpha 1C subunit1, calcium channel L type, alpha 1 polypeptide isoform 1 cardiac muscle, calcium channel cardiac dihydropyridine-sensitive alpha-1 subunit, voltage-gated L-type calcium channel Cav1.2 alpha 1 subunit splice variant 10
应用稀释比例: WB (1:1000), IHC-P (1:1000), ICC/IF (1:100), IP (1:200)
应用说明: 1 µg/ml was sufficient for detection of Cav1.2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
免疫原: Fusion protein amino acids 1507-1733 (intracellular carboxyl terminus) of rabbit Cav1.2
分子量: 240kDa
防腐剂: PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.
纯化方法: Protein G Purified
特异性: Detects ~240kDa (varies with cell background due to glycosylation).
研究领域: Neuroscience
UniProt ID: P15381
NCBI 参考序列: NP_001129994.1
Entrez 基因 ID: 100101555
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
产品子类型: Recombinant Antibody
描述: Mouse Anti-Rabbit Cav1.2 Monoclonal IgG1
克隆号: S57
别名: Voltage-dependent L-type calcium channel subunit alpha-1C, Cav1.2, Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle, Voltage-gated calcium channel subunit alpha Cav1.2, DHPR alpha-1 subunit, CACH3, CACN4, CACNL1A2, alpha-1 subunit voltage-dependent calcium channel, calcium channel voltage-dependent L type alpha 1C subunit1, calcium channel L type, alpha 1 polypeptide isoform 1 cardiac muscle, calcium channel cardiac dihydropyridine-sensitive alpha-1 subunit, voltage-gated L-type calcium channel Cav1.2 alpha 1 subunit splice variant 10
应用稀释比例: WB (1:1000), IHC-P (1:1000), ICC/IF (1:100), IP (1:200)
应用说明: 1 µg/ml was sufficient for detection of Cav1.2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
免疫原: Fusion protein amino acids 1507-1733 (intracellular carboxyl terminus) of rabbit Cav1.2
分子量: 240kDa
防腐剂: PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.
纯化方法: Protein G Purified
特异性: Detects ~240kDa (varies with cell background due to glycosylation).
研究领域: Neuroscience
UniProt ID: P15381
NCBI 参考序列: NP_001129994.1
Entrez 基因 ID: 100101555
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.2 Monoclonal Antibody, Clone S57. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav1.2 Antibody (D) Composite.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.2 Monoclonal Antibody, Clone S57. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-Cav1.2 Antibody. (C) Composite. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cav1.2 Antibody. (D) Composite.
Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57. Tissue: hippocampus. Species: Human. Fixation: 10% formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Western Blot analysis of Hamster T-CHO cell lysate showing detection of CaV1.2 Calcium Channel protein using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody at 1:1000.
Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody, Clone S57. Tissue: Brain Tissue. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Magnification: 40x.
Immunoprecipitation analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57. Tissue: INS-1E cells. Species: Rat. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody at 1:200.
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文献和实验相关实验
to 1 liter with Qcheck to make sure pH is 3.0(2) Affigel Blue Running Buffer10 mM K2HPO4 2.28 g K2HPO40.15 M NaCl 8.2 g NaCl0.02% azide 0.2 g Na azideup to 1 liter with Q(3) 1.4 M NaCl8.1 g NaClup to 100 ml with Q(4) Saturated NH4SO4767 g NH4SO4add 1 liter Q
mls of PBS. Put 200 ul (3 mg/ml) of the antibody to an eppendorf tube. Add 0.8 mls of PBS. Pellet 1 ml of the cell suspension and discard the PBS supernatant. Add the antibody solution to the cell pellet, gently resuspend the cells
Antibody Staining of Ovaries 1. Dissect ovaries in EBR 2. Transfer ovaries into an Eppendorf tube and add 100 µl devitellinizing buffer and 600-µl heptane 3. Agitate gently for 10 minutes
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Cav1.2 Antibody 抗体，orb67390，Biorbyt
￥6903
