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- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
12 months from date of receipt.
- 英文名:
Aminoallyl-dUTP-6-JOE
- 库存:
99
- 供应商:
Biorbyt
- 规格:
120 ul
描述: Aminoallyl-dUTP conjugated to 6-JOE.
别名: 5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with 6-JOE, Triethylammonium salt
浓度: 1.0 mM-1.1 mM
形态: solution in water; pH: 7.5 ± 0.5
应用说明: Spectroscopic Propertie: λexc 520 nm, λem 548 nm, ε 75.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
分子式: C35H32Cl2N3O22P3
分子量: Theoretical MW: 1010.47 g/mol (free acid); Detected MW: 1010.01 g/mol (free acid)
纯度: ≥ 95% (HPLC)
SMILES: C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)c1cc2C3(c4cc(c(O)c(Cl)c4Oc4c(Cl)c(O)c(cc34)OC)OC)OC(=O)c2cc1
保存条件: store at -20 °C
备注: For research use only.
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文献和实验Protocol for Reverse Transcription and Amino-allyl Coupling of RNA
DTT Superscript II RT (Gibco) RNasin (Gibco, optional) 0.1M 200U/mL 40U/mL 6.0 mL 0.6 3.0 1.5 0.5 50X dNTP stock solution using a 4:1 ratio aminoallyl-dUTP to dTTP***: 10 mL each 100 mM
Protocol for Reverse Transcription and Amino-allyl Coupling of RNA
3.0 1.5 0.5 50X dNTP stock solution using a 4:1 ratio aminoallyl-dUTP
Amino-allyl Reverse Transcription
: FOR 2:3 10uL each 100mM dATP,dGTP,dCTP, 4uL 100mM aa-dUTP*, 6uL 100 mMdTTP * 5-(3-Aminoallyl)-2'-deoxyuridine 5'-triphosphate sodium salt, Sigma-Aldrich Catalog A-0410 cDNA synthesis Concentration
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