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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商:
西宝
- 库存:
大量
- 英文名:
Anti-Digoxigenin-AP
- 规格:
150 U (200 µl)
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文献和实验Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
In Situ Hybridization Using Digoxigenin Labeled Probes
dilution of Anti Digoxigenin-POD (Boehringer Cat. #1207 733). 1) Wash the slides in 1X PBS 2 times for 5 minutes each at room temperature 2) Wash for 2 minutes in 1X PBS/0.1 % Triton-X-100. 3) Rinse in 1X PBS 4) Place the slides horizontally
96-well RNA In Situ Hybridization Protocol
+ 5% Normal Goat Serum + anti-digoxigenin Alkaline Phosphatase (1:2000 dilution), rock 2 hrs at RT. 20. Rinse twice with PBT . 21. Wash embryos 9 X for 10 min. in PBT. 22. Rinse twice with 200ul Alkaline Phosphatase buffer (AP buffer). 23
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