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HIF3 alpha Rabbit Polyclonal A

ntibody抗体,orb101652,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb101652
  • 英国
  • 2025年12月03日
  • ELISA, ICC, IF, IHC-Fr, IHC-P
  • Rabbit
  • Human, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      HIF3 alpha Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      HIF3 alpha Rabbit Polyclonal Antibody

    • 靶点

      HIF3A

    • 浓度

      1mg/ml

    • 应用范围

      ELISA, ICC, IF, IHC-Fr, IHC-P

    • 宿主

      Rabbit

    • 适应物种

      Human, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb101652

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human HIF3 alpha (131-230/669aa)

    • 规格

      50 ul

    产品描述:HIF3 alpha Rabbit Polyclonal Antibody

    别名:HIF 3 alpha; HIF-3 alpha; HIF 3A; HIF 3A4; HIF3 alpha; HIF3A; HIF 3A; HIF-3A; Hypoxia Inducible Factor 3 alpha; Hypoxia inducible factor 3 alpha subunit; Hypoxia inducible factor three alpha; Inhibitory PAS domain protein; IPAS; MOP7; PASD7.

    免疫原:KLH conjugated synthetic peptide derived from human HIF3 alpha (131-230/669aa)

    预测反应性:Bovine, Canine, Equine, Mouse, Porcine

    分子量:74 kDa

    应用稀释比例:IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, ELISA=1:5000-10000

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:Q9Y2N7

    Note:For research use only.

    产品细节图片1
    HUVEC cell, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HIF3 alpha) polyclonal Antibody, Unconjugated (orb101652) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

    产品细节图片2
    Fixative-fixed, paraffin embedded (Rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HIF3 alpha) Polyclonal Antibody, Unconjugated (orb101652) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片3
    Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-HIF3 alpha (orb101652) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 74 kD, Observed band size: 75 kD.

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    图标文献和实验
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    • Antigen Design Sera Purification Tech Sheet

      for antibody generation. Algorithms for predicting protein characteristics such as hydrophilicity/hydrophobicity and secondary structure regions such as alpha-helix, beta-sheet and beta-turn aid selection of a potentially exposed, immunogenic internal

    • Antigen Design Sera Purification Tech Sheet

      should not be included in the sequence selected for antibody generation. Algorithms for predicting protein characteristics such as hydrophilicity/hydrophobicity and secondary structure regions such as alpha-helix, beta-sheet and beta-turn aid selection

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