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CD16 Rabbit Polyclonal Antibod

y抗体,orb101668,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb101668
  • 英国
  • 2025年12月03日
  • WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      CD16 Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      CD16 Rabbit Polyclonal Antibody

    • 靶点

      FCGR3A

    • 浓度

      1mg/ml

    • 应用范围

      WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb101668

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human IGFR3/CD16 (131-230/254aa)

    • 规格

      50 ul

    产品描述:CD16 Rabbit Polyclonal Antibody

    别名:FCG3A_HUMAN; Low affinity immunoglobulin gamma Fc region receptor III-A; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; CD_antigen: D16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3.

    免疫原:KLH conjugated synthetic peptide derived from human IGFR3/CD16 (131-230/254aa)

    预测反应性:Bovine, Porcine, Rabbit, Sheep

    分子量:27 kDa

    应用稀释比例:WB=1:500-1000

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P08637

    Note:For research use only.

    产品细节图片1
    Cell: U937 (4% Fixative fixed for 10 minutes, 2% BSA at 4∑blocked for 30 minutes). Concentration: 1:100, Incubation: 40 minutes. Flow cytometric analysis of Rabbit Anti-CD16 antibody (orb101668) (green) compared with control in the absence of primary antibody (blue) followed by U937. Secondary antibody: Goat Anti-rabbit IgG/PE antibody.

    产品细节图片2
    Fixative-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD16) Polyclonal Antibody, Unconjugated (orb101668) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片3
    Sample: Lane 1: Thymus (Rat) Lysate at 40 ug, Lane 2: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-CD16 (orb101668) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 60 kD.

    产品细节图片4
    Sample: Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-CD16 (orb101668) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 50 kD.

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    图标文献和实验
    相关实验
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        Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation.     Rabbit Immunization Initial Preparation Your antigen

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