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- 2026年01月09日
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西宝生物
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文献和实验Adult cattle serum as supplemental nutrient in lymphocyte cultures for chromosomes studies
fetal calf serum (FCS), which has specialty being free of antibodies. However, besides cost these days various ethical issues on its production procedure are cropping up in the society. In addition there are a few scientific drawbacks of the use of FCS
Establishment of a Primary Culture
Phosphate buffered saline (PBS) Trypsin, cold sterilized in a 125 ml sterile erlenmeyer containing a magnetic stirring bar Minimum Essential Medium Fetal Calf Serum Clinical centrifuge with sterile capped
Virus Infection of Fibroblasts
Procedure: Day One 1. Plate cells in 60 mm dishes using 5 ml DMEM containing 10% Calf Serum. The protocol has worked well for NIH3T3 with 1 x 105 cells and BALB/c3T3 with 2 x 105 cells. For the NIH cell line use 10% fetal calf serum
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