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- SH30070.03T
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- 2026年01月09日
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西宝生物
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文献和实验media: Ap 50 μg/ml, Km 50 μg/ml. For BACs and low copy vectors, on plates and in liquid media: chloramphenicol (Cm) 20 μg/ml, Ap 50 μg/ml and Km 15 μg/ml. Tetracycline (Tc) concentrations for selection of pSC101BADγβαA[tet] was 5 and 3 μg/ml on plates
Pulse-Chase mRNA decay assay in HeLa Tet-off cells:
to break up cell clusters to ensure single cells. Dilute freshly split cell suspension 1:12 or 1:15 (depending on % confluency) in DMEM/10% FBS in a 50 ml conical in a total volume equal to or more than the number of wells to be plated (for e.g. make
The Cell Environment (including types of culture medium)
since suppliers of large quantities of serum perform a battery of quality control tests and supply a certificate of analysis with the serum. In particular serum is screened for the presence of bovine viral diarrhoea virus (BVDV) and mycoplasma. Heat inactivation
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