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TLR9 Rabbit Polyclonal Antibod

y抗体,orb7097,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb7097
  • 英国
  • 2025年12月03日
  • FC, ICC, IF, IHC-Fr, IHC-P
  • Rabbit
  • Human, Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      TLR9 Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      TLR9 Rabbit Polyclonal Antibody

    • 靶点

      TLR9

    • 浓度

      1mg/ml

    • 应用范围

      FC, ICC, IF, IHC-Fr, IHC-P

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb7097

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human TLR9 (951-1032/1032aa)

    • 规格

      50 ul

    产品描述:TLR9 Rabbit Polyclonal Antibody

    别名:Toll-like receptor 9; CD 289; CD289; TLR 9; Toll like receptor 9; Toll like receptor 9 isoform A precursor; Toll like receptor 9 isoform B; TLR9_HUMAN.

    免疫原:KLH conjugated synthetic peptide derived from human TLR9 (951-1032/1032aa)

    预测反应性:Bovine, Canine, Equine, Porcine, Rat, Sheep

    分子量:113 kDa

    应用稀释比例:IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:Q9NR96

    Note:For research use only.

    产品细节图片1
    A549 cell, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (TLR9) polyclonal Antibody, Unconjugated (orb7097) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

    产品细节图片2
    Blank control (blue): Raji (fixed with 2% Fixative (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-TLR9/CD289 antibody (orb7097), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

    产品细节图片3
    Tissue/Cell: mouse embryo tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TLR9/CD289 Polyclonal Antibody, Unconjugated (orb7097) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    图标文献和实验
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