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S100-A8 / MRP8 Rabbit Polyclon

al Antibody抗体，orb6912，biorbyt
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  • ￥3120
  • biorbyt已认证
  • orb6912
  • 英国
  • 2025年12月03日
  • IF, IHC-Fr, IHC-P, IP, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      S100-A8 / MRP8 Rabbit Polyclonal Antibody抗体

    • 抗体英文名：

      S100-A8 / MRP8 Rabbit Polyclonal Antibody

    • 靶点：

      S100A8

    • 浓度：

      1mg/ml

    • 应用范围：

      IF, IHC-Fr, IHC-P, IP, WB

    • 宿主：

      Rabbit

    • 适应物种：

      Human, Mouse, Rat

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb6912

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 形态：

      Liquid

    • 亚型：

      IgG

    • 免疫原：

      KLH conjugated synthetic peptide derived from mouse S100A8 (25-89/89aa)

    • 规格：

      50 ul

    产品描述：S100-A8 / MRP8 Rabbit Polyclonal Antibody

    别名:60B8Ag; AI323541; B8Ag; BEE11; CAGA; Calgranulin-A; Calprotectin L1L subunit; Calprotectin, included; CFAG; CGLA; Chemotactic cytokine CP-10; CP-10; Cystic fibrosis antigen; L1Ag; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP-8; Myeloid-related protein 8; Neutrophil cytosolic 7 kDa protein; NIF; p8; Pro-inflammatory S100 cytokine; Protein S100-A8; S100 calcium binding protein A8(calgranulin A); S100 calcium binding protein A8; S100 calcium-binding protein A8; S100A8; S100A8/S100A9 complex, included; S10A8_MOUSE; Urinary stone protein band A.

    免疫原：KLH conjugated synthetic peptide derived from mouse S100A8 (25-89/89aa)

    预测反应性:Rat

    分子量:10 kDa

    应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, IP=1:20-50

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P27005

    Note:For research use only.

    产品细节图片1
    Blank control: K562. Primary Antibody (green line): Rabbit Anti-S100-A8 antibody (orb6912), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-S100-A8 antibody (orb6912), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片3
    Blank control: Mouse spleen. Primary Antibody (green line): Rabbit S100-A8/MRP8/FITC Conjugated antibody, dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

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    图标文献和实验
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    • Preparation of Polyclonal Antibodies to Retinoid Receptors

      of a retinoid “X” receptor α (RXRα) antibody on Western blots contributed to the identification of the RXR ligand by detection of the receptor in fractions of whole-cell lysates that were able to bind the 9-cis retinoic acid ligand (2 ) Antibodies

    • Indirect Immunofluorescence

      Reagents:  NGS-Normal Goat Serum  PBST-0.5% Tween-20/CMF-PBS  CMF-PBS  1°Ab-mouse MF20 (anti-myosin monoclonal)  1°Ab-rabbit myoD (anti-myoD polyclonal)  2°Ab-goat anti-mouse IgG-FITC  2°Ab-goat anti-rabbit IgG-FITC

    • p27 ELISA

      then serially diluted in 100 µl assay buffer on the plate). Incubate @ RT for 1 hr while shaking. Meanwhile let TMB solution equilibrate to RT. Wash plate 3x and add 100 µl/well rabbit polyclonal anti-p27 antibody diluted 1:1000 in assay buffer

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