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Phospho-PI3 kinase p85 alpha +

gamma (Tyr467 + Tyr199) Rabbit Polyclonal Antibody抗体,orb106105,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb106105
  • 英国
  • 2025年12月03日
  • FC, IF, IHC-Fr, IHC-P
  • Rabbit
  • Human, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      Phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) Rabbit Polyclonal Antibody

    • 靶点

      PIK3R1

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P

    • 宿主

      Rabbit

    • 适应物种

      Human, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb106105

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated Synthesised phosphopeptide derived from human PI3 kinase p85 alpha around the phosphorylation site of Tyr467 RL(p-Y)EE

    • 规格

      50 ul

    产品描述:Phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) Rabbit Polyclonal Antibody

    别名:PI3 kinase p85 alpha(phospho Tyr467); PI3 kinase p55 gamma(phospho Tyr199); phospho-PI3 kinase p85 alpha/gamma(Tyr467/Tyr199); PI3-kinase p85 subunit alpha; PI3-kinase p85 subunit gamma; P85A_HUMAN; Phosphatidylinositol 3-kinase regulatory subunit alpha; GRB1; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha(PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha); PI 3-kinase p85α; PI 3-kinase p85 α; PI 3-kinase p85-α; SH3_PI3K_p85alpha; PI3-kinase p85 subunit alpha; phosphoinositide-3-kinase regulatory subunit 1; p85; AGM7; IMD36; p85-ALPHA; PI 3 Kinase p85 alpha;

    免疫原:KLH conjugated Synthesised phosphopeptide derived from human PI3 kinase p85 alpha around the phosphorylation site of Tyr467 RL(p-Y)EE

    预测反应性:Bovine, Canine, Equine, Mouse, Porcine, Rabbit

    分子量:85/55 kDa

    应用稀释比例:IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Pubmed:31159591, 32050796, 31282213, 28790168, 30255285

    UniProt ID:P27986

    Note:For research use only.

    产品细节图片1
    Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199)/FITC Conjugated antibody, Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Tissue/Cell: rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-PI3 kinase p85 alpha+gamma (Tyr467+Tyr199) Polyclonal Antibody, Unconjugated (orb106105) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    图标文献和实验
    该产品被引用文献
    Chen, Yanbo et al. Pretreatment of ghrelin protects H9c2 cells against hypoxia/reoxygenation-induced cell death via PI3K/AKT and AMPK pathways Artif Cells Nanomed Biotechnol, 47, 2179-2187 (2019), Deng, Dan et al. Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21 Artif Cells Nanomed Biotechnol, 48, 610-619 (2020), Wang, Baoying et al. Puerarin attenuates hypoxia-resulted damages in neural stem cells by up-regulating microRNA-214 Artif Cells Nanomed Biotechnol, 47, 2746-2753 (2019), Zhang, Kexiang et al. MicroRNA-211/BDNF axis regulates LPS-induced proliferation of normal human astrocyte through PI3K/AKT pathway Biosci Rep, 37, (2017), Wang, Yanan et al. Luteolin Partially Inhibits LFA-1 Expression in Neutrophils Through the ERK Pathway Inflammation, 42, 365-374 (2019)
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    文献支持
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    ¥3120