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- 2025年12月28日
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文献和实验电镜样品制备方法 Specimen preparation for electron microscopy
2. (May be stored overnight at this stage if absolutely necessary.) Dehydrate in 90% ethanol - 10 mins. x 2. Dehydrate in 100% ethanol - 20 mins. x 2. Propylene oxide (1.2 epoxy propane) - 10 mins. x 2. Propylene oxide/epoxy resin
.) Dehydrate in 90% ethanol - 10 mins. x 2. Dehydrate in 100% ethanol - 20 mins. x 2. Propylene oxide (1.2 epoxy propane) - 10 mins. x 2. Propylene oxide/epoxy resin mixture (50/50) - 1 hour. Epoxy resin - overnight
Site-directed hydroxy radical mapping of nucleosome positions in vitro
Millipore Amicon Ultra 5000 MWCO and Centricon YM30 concentrators. Multivalent metal ions can be removed by BioRad Chelex 100 (200-400 mesh) resin. Sequencing gel solutions are SequaFlowgel 6 and 8 (cat H18049 and H18051) from Flowgen UK. Millipore Stericup
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