- ￥3120
- biorbyt
- orb6351
- 英国
- 2025年12月03日
- FC, IF, IHC-Fr, IHC-P, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 技术资料
- 抗体名：
Phospho-DAXX (Ser517) Rabbit Polyclonal Antibody抗体
- 抗体英文名：
Phospho-DAXX (Ser517) Rabbit Polyclonal Antibody
- 靶点：
DAXX
- 浓度：
1mg/ml
- 应用范围：
FC, IF, IHC-Fr, IHC-P, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb6351
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated Synthesised phosphopeptide derived from human DAXX around the phosphorylation site of Ser517 SR(p-S)SG
- 规格：
50 ul
产品描述：Phospho-DAXX (Ser517) Rabbit Polyclonal Antibody
别名:BING 2; BING2; DAP 6; DAP6; Death associated protein 6; Death domain associated protein 6; EAP 1; EAP1; ETS1 associated protein 1; Fas death domain associated protein; hDaxx; MGC126245; MGC126246; DAXX_HUMAN.
免疫原：KLH conjugated Synthesised phosphopeptide derived from human DAXX around the phosphorylation site of Ser517 SR(p-S)SG
预测反应性:Porcine
分子量:81 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:Q9UER7
Note:For research use only.
别名:BING 2; BING2; DAP 6; DAP6; Death associated protein 6; Death domain associated protein 6; EAP 1; EAP1; ETS1 associated protein 1; Fas death domain associated protein; hDaxx; MGC126245; MGC126246; DAXX_HUMAN.
免疫原：KLH conjugated Synthesised phosphopeptide derived from human DAXX around the phosphorylation site of Ser517 SR(p-S)SG
预测反应性:Porcine
分子量:81 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:Q9UER7
Note:For research use only.
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-phospho-DAXX (Ser517) antibody (orb6351), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Fixative-fixed, paraffin embedded (rat breast), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (phospho-DAXX (Ser517)) Polyclonal Antibody, Unconjugated (orb6351) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: K562 (Human) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Lane 3: Panc-1 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-DAXX (Ser517) (orb6351) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 110 kD, Observed band size: 110 kD.
Tissue/Cell: mouse kidney tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-DAXX (Ser517) Polyclonal Antibody, Unconjugated (orb6351) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
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Phospho-DAXX (Ser517) Rabbit Polyclonal Antibody抗体，orb6351，biorbyt
￥3120
