- ￥3120
- biorbyt
- orb5765
- 英国
- 2025年12月03日
- FC, IF, IHC-Fr, IHC-P, WB
- Rabbit
- Human, Mouse
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- 详细信息
- 技术资料
- 抗体名：
Legumain Rabbit Polyclonal Antibody抗体
- 抗体英文名：
Legumain Rabbit Polyclonal Antibody
- 靶点：
LGMN
- 浓度：
1mg/ml
- 应用范围：
FC, IF, IHC-Fr, IHC-P, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb5765
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human Legumain (201-300/433aa)
- 规格：
50 ul
产品描述：Legumain Rabbit Polyclonal Antibody
别名:AEP; Asparaginyl endopeptidase; EC 3.4.22.34; LGMN; LGMN1; Protease, cysteine 1; protease, cysteine, 1(legumain); PRSC1; LGMN_HUMAN.
免疫原：KLH conjugated synthetic peptide derived from human Legumain (201-300/433aa)
预测反应性:Bovine, Canine, Equine, Rat
分子量:46 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:Q99538
Note:For research use only.
别名:AEP; Asparaginyl endopeptidase; EC 3.4.22.34; LGMN; LGMN1; Protease, cysteine 1; protease, cysteine, 1(legumain); PRSC1; LGMN_HUMAN.
免疫原：KLH conjugated synthetic peptide derived from human Legumain (201-300/433aa)
预测反应性:Bovine, Canine, Equine, Rat
分子量:46 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:Q99538
Note:For research use only.
Blank control: HL60. Primary Antibody (green line): Rabbit Anti-Legumain antibody (orb5765), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Overlay histogram showing Mouse spleen cells (red line). The cells were fixed with 1% Fixative (10 min). The cells were then incubated with the antibody (2 ug/1x106 cells) for 30 min at 22-25°C. Isotype control antibody (black line) was rabbit IgG (2 ug/1x106 cells) used under the same conditions. Acquisition of events were used for analysis.
Fixative-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Legumain) Polyclonal Antibody, Unconjugated (orb5765) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-Legumain (orb5765) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46 kD, Observed band size: 55 kD.
Sample: Lane 1: Human JAR cell lysates, Lane 2: Human MCF-7 cell lysates, Primary: Anti-Legumain (orb5765) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46 kDa, Observed band size: 55 kDa.
Sample: Placenta (Mouse) Lysate at 40 ug, Primary: Anti-Legumain (orb5765) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46 kD, Observed band size: 55 kD.
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Legumain Rabbit Polyclonal Antibody抗体，orb5765，biorbyt
￥3120
