Phospho-HNF4 (Ser313) Rabbit Polyclonal Antibody抗体,orb106148,biorbyt

Phospho-HNF4 (Ser313) Rabbit P

olyclonal Antibody抗体,orb106148,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb106148
  • 英国
  • 2025年12月03日
  • FC, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 技术资料
    • 抗体名

      Phospho-HNF4 (Ser313) Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      Phospho-HNF4 (Ser313) Rabbit Polyclonal Antibody

    • 靶点

      HNF4A

    • 浓度

      1mg/ml

    • 应用范围

      FC, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb106148

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated Synthesised phosphopeptide derived from human HNF4 alpha around the phosphorylation site of Ser313 LR(p-S)QV

    • 规格

      50 ul

    产品描述:Phospho-HNF4 (Ser313) Rabbit Polyclonal Antibody

    别名:HNF4(phospho S313); p-HNF4(phospho S313); Hepatic nuclear factor 4 alpha; Hepatocyte nuclear factor 4 alpha; Hepatocyte nuclear factor 4; HNF 4 alpha; HNF 4; HNF4; HNF4A; HNF4a7; HNF4a8; HNF4a9; Hnf4alpha; MODY 1; MODY; MODY1; NR2A1; NR2A21; Nuclear receptor subfamily 2 group A member 1; TCF 14; TCF; TCF14; Tcf4; Transcription factor 14, hepatic nuclear factor; Transcription factor 14; Transcription factor HNF 4; Transcription factor HNF4.

    免疫原:KLH conjugated Synthesised phosphopeptide derived from human HNF4 alpha around the phosphorylation site of Ser313 LR(p-S)QV

    预测反应性:Bovine, Equine, Gallus, Guinea pig, Porcine, Rabbit

    分子量:52 kDa

    应用稀释比例:WB=1:500-2000, Flow-Cyt=2ug/test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P41235

    Note:For research use only.

    Phospho-HNF4 (Ser313) Rabbit P
    Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-phospho-HNF4 (Ser313) antibody (orb106148), Dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    Phospho-HNF4 (Ser313) Rabbit P
    Blank control (black line): HUVEC. Primary Antibody (green line): Rabbit Anti-phospho-HNF4 (Ser313) antibody (orb106148), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    Phospho-HNF4 (Ser313) Rabbit P
    Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Mouse Stomach tissue lysates, Lane 3: Rat Stomach tissue lysates, Primary: Anti-phospho-HNF4 (Ser313) (orb106148) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kDa, Observed band size: 50 kDa.

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