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TSPO/PBR Rabbit Polyclonal Ant

ibody抗体,orb5845,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb5845
  • 英国
  • 2025年12月03日
  • FC, IF, IHC-Fr, IHC-P
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      TSPO/PBR Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      TSPO/PBR Rabbit Polyclonal Antibody

    • 靶点

      TSPO

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb5845

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human PBR (31-130/169aa)

    • 规格

      50 ul

    产品描述:TSPO/PBR Rabbit Polyclonal Antibody

    别名:BPBS; BZRP; DBI; IBP; Isoquinoline carboxamide-binding protein; MBR; mDRC; Mitochondrial benzodiazepine receptor; PBR; PBS; Peripheral benzodiazepine receptor; Peripheral benzodiazepine receptor-related protein; Peripheral type benzodiazepine receptor; pk18; PKBS; PTBR; Ptbzr; PTBZR02; RATPTBZR02; translocator protein(18kDa); Translocator protein; TSPO; Tspo1; Benzodiazapine receptor(peripheral); Benzodiazepine peripheral binding site.

    免疫原:KLH conjugated synthetic peptide derived from human PBR (31-130/169aa)

    预测反应性:Canine, Porcine

    分子量:18 kDa

    应用稀释比例:IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Pubmed:33664474

    UniProt ID:B1AH88

    Note:For research use only.

    产品细节图片1
    Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-TSPO/PBR antibody (orb5845), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Fixative-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TSPO/PBR) Polyclonal Antibody, Unconjugated (orb5845) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片3
    Fixative-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PBR) Polyclonal Antibody, Unconjugated (orb5845) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片4
    Fixative-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PBR) Polyclonal Antibody, Unconjugated (orb5845) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

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    图标文献和实验
    该产品被引用文献
    Frison, Michele et al. The translocator protein (TSPO) is prodromal to mitophagy loss in neurotoxicity Mol Psychiatry, (2021)
    相关实验
    • Polyclonal Antibody Production

      Zealand White Rabbits and have them delivered to the ARC. Don’t put all your eggs in one basket only using one rabbit. Let them get accustomed to there home there for two weeks before the first pre-bleed. Pre-bleed        

    • Polyclonal Antibody Production

        Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation.     Rabbit Immunization Initial Preparation Your antigen

    • Preparation of Polyclonal Antibodies to Retinoid Receptors

      of a retinoid “X” receptor α (RXRα) antibody on Western blots contributed to the identification of the RXR ligand by detection of the receptor in fractions of whole-cell lysates that were able to bind the 9-cis retinoic acid ligand (2 ) Antibodies

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    文献支持
    TSPO/PBR Rabbit Polyclonal Antibody抗体,orb5845,biorbyt
    ¥3120